https://doi.org/10.4081/bse.113
Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples
Published: 14 February 2020
Abstract Views: 1057
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The aim of this study was to apply a workflow, integrating delipidation methods and advanced 3D imaging techniques for mapping of the global neuronal organization of brain organoids. These are self-organizing constructs in vitro generated from human pluripotent stem cells encased in a Matrigel shell, which resemble downscaled structural and functional features of human brains. In particular, we focused on midbrain organoids, widely considered a promising tool for studying dopaminergic neuron degeneration in Parkinson’s Disease. The evaluation of the microanatomical alterations at a patient-level will potentially guide future research of this neuropathy, providing meaningful human specific data in line with the European Directives and the 3Rs principles.
How to Cite
Magliaro, C., & Ahluwalia, A. (2020). Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples. Biomedical Science and Engineering, 1(1). https://doi.org/10.4081/bse.113
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