https://doi.org/10.4081/bse.113
Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples
Published: 14 February 2020
Abstract Views: 1057
PDF: 439
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The aim of this study was to apply a workflow, integrating delipidation methods and advanced 3D imaging techniques for mapping of the global neuronal organization of brain organoids. These are self-organizing constructs in vitro generated from human pluripotent stem cells encased in a Matrigel shell, which resemble downscaled structural and functional features of human brains. In particular, we focused on midbrain organoids, widely considered a promising tool for studying dopaminergic neuron degeneration in Parkinson’s Disease. The evaluation of the microanatomical alterations at a patient-level will potentially guide future research of this neuropathy, providing meaningful human specific data in line with the European Directives and the 3Rs principles.
How to Cite
Magliaro, C., & Ahluwalia, A. (2020). Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples. Biomedical Science and Engineering, 1(1). https://doi.org/10.4081/bse.113
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Most read articles by the same author(s)
- Ludovica Cacopardo, Joana Costa, Nicole Guazzelli, Serena Giusti, Sandro Meucci, Alessandro Corti, Giorgio Mattei, Arti Ahluwalia, Real-time cellular impedance monitoring and imaging in a dual-flow bioreactor , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Licia Di Pietro, Alice Ravizza, Giovanni Vozzi, Andrés Diaz Lantada, Arti Ahluwalia, Carmelo De Maria, European regulatory framework for the clinical translation of bioprinted scaffolds and tissues , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Nicole Guazzelli, Ludovica Cacopardo, Arti Ahluwalia, Engineering magneto-responsive core-shell microbeads for mimicking peristalsis and alveolar breathing in vitro , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- Roberta Nossa, Joana Costa, Ludovica Cacopardo, Arti Ahluwalia, Engineering a dynamic model of the alveolar interface for the study of aerosol deposition , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Piera Mancini, Ermes Botte, Chiara Magliaro, Arti Ahluwalia, Toward scalable in vitro models: a novel experimental and computational pipeline for the identification of cellular metabolic parameters , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- Ermes Botte, Pietro Vagaggini, Joana Costa, Lara Faccani, Ilaria Zanoni, Anna Costa, Arti Ahluwalia, A novel in silico tool for dose assessment in cell culture nanotoxicology , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- Arti Ahluwalia, The Italian Centro 3R: Mission and early achievements presented during the Second Annual Meeting , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
Similar Articles
- Ryan Conder, Leon H. Chew, Fisal Elstone, Marianne Lankhorst, Adam Añonuevo, Salvatore Simmini, Wing Chang, Allen C. Eaves, Terry E. Thomas, Sharon A. Louis, Organoid culture systems: Products supporting one of the biggest revolutions in biological research , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Marco Campisi, Sharon W. L. Lee, Tatsuya Osaki, Luca Possenti, Clara Mattu, Giulia Adriani, Valeria Chiono, Roger D. Kamm, In vitro microfluidic modelling of the human blood-brain-barrier microvasculature and testing of nanocarrier transport , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Emanuele Gasparotti, Margherita Cioffi, Vincenzo Positano, Emanuele Vignali, Benigno Marco Fanni, Katia Capellini, Dorela Haxhiademi, Emiliano Costa, Sergio Berti, Luigi Landini, Simona Celi, Laura Gribaldo, Analysis of non-animal methods and models for research in cardiovascular disease , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Andrea Cacciamali, Chiara Romano, Martina Angela Checco, Riccardo Villa, Detection of pathogens from environmental samples for the evaluation of the health status of laboratory animals , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- Erika Molica Colella, Silvia Dotti, Riccardo Villa, Guerino Lombardi, Massimo Amadori, Application of two in vitro methods for the toxicity test of autogenous vaccines , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Ludovica Cacopardo, Joana Costa, Nicole Guazzelli, Serena Giusti, Sandro Meucci, Alessandro Corti, Giorgio Mattei, Arti Ahluwalia, Real-time cellular impedance monitoring and imaging in a dual-flow bioreactor , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Claudio Conci, Emanuela Jacchetti, Tommaso Zandrini, Laura Sironi, Maddalena Collini, Giuseppe Chirico, Giulio Cerullo, Roberto Osellame, Manuela Teresa Raimondi, Quantification of the foreign body reaction by means of a miniaturized imaging window for intravital nonlinear microscopy , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Salvatore Simmini, Allen C. Eaves, Sharon A. Louis, Wing Chang, Human pluripotent stem cell-derived organoids and their potential to reduce the use of animal models in research , Biomedical Science and Engineering: Vol. 3 No. 1 (2022)
- G. Terribile, S. Di Girolamo, E. Donzelli, F. Re, P. Gasco, G. Sancini, The fantastic voyage of solid lipid nanoparticles from the lung to the brain: non-invasive tomographic imaging as a feasible refinement process , Biomedical Science and Engineering: Vol. 4 No. 1 (2023)
- Silvia Bolognin, Lisa M. Smits, Sarah L. Nickels, Stefano Magni, Paul Antony, Kamil Grzyb, Rejko Krüger, Alexander Skupin, Jens C. Schwamborn, A new brain organoid model to study Parkinson's disease , Biomedical Science and Engineering: Vol. 3 No. 1 (2022)
You may also start an advanced similarity search for this article.
