Macrophage supernatant infected with Leishmania major mediates the cytology of fibroblast cells in skin wounds

  • Mohsen Ghomashlooyan Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of. https://orcid.org/0000-0001-8445-4162
  • Fatemeh Namdar Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of. https://orcid.org/0000-0003-4172-4315
  • Mehrafarin Fesharaki Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of.
  • Shervin Ghaffari Hosseini Cardiac Rehabilitation Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of.
  • Manizheh Narimani Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of.
  • Zahra Sebghatollahi Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of.
  • Bahareh Basirpour Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of.
  • Seyed Hossein Hejazi | hejazi@med.mui.ac.ir Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, Islamic Republic of. https://orcid.org/0000-0002-3733-1220

Abstract

Objective: Healing of Cutaneous Leishmaniasis relies on the effective and modulates protective immune responses. Although the immune system is necessary to eliminate the parasite, it could be considered as the main cause of ulcers. Therefore, main aim of this study was to explore the possible regulatory functions of macrophage supernatant infected with Leishmania major on the fibroblast cells.

Materials and Methods: In this experimental study, different concentrations of infected macrophage supernatant extract (50, 100, 150, 200, and 250μg/mL) were tested at different times (6, 24, 48, and 72h) and the effect of the leishmanicidal extract on fibroblast cells was determined by MTS assay. Also, the flow-cytometry technique was used for the investigation of apoptosis induction percentage.

Results: MTS assay showed that the leishmanicidal effect of infected macrophage supernatant extract was dependent on the concentration and the time of treatment. So, the best efficacy was observed in 200 μg/mL with 72 hours exposure time. Flow cytometry analysis showed that the infected macrophage supernatant extract could induce apoptosis in cultured fibroblasts.

Conclusions: We have demonstrated that reduction of survival rate and induction of apoptosis in fibroblasts displayed a similar manner to keratinocytes when exposed to infected macrophages with L. major. Our data suggest that such a phenomenon can be the underlying cause of lesions with scarring, and future, the mechanism remains to be elucidated.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2020-12-02
Info
Issue
Section
Research Articles
Keywords:
Fibroblast cell, Leishmania major, Apoptosis, Flow cytometry, MTT assay
Statistics
  • Abstract views: 126

  • PDF: 84
How to Cite
Ghomashlooyan, M., Namdar, F., Fesharaki, M., Hosseini, S. G., Narimani, M., Sebghatollahi, Z., Basirpour, B., & Hejazi, S. H. (2020). Macrophage supernatant infected with <em>Leishmania major</em&gt; mediates the cytology of fibroblast cells in skin wounds. Infectious Diseases and Herbal Medicine, 1(1). https://doi.org/10.4081/idhm.2020.83

Most read articles by the same author(s)