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Evaluation of antioxidant and neuropharmacological properties of Leea aequata leaves

Authors

Md. Riaz Hossain
https://orcid.org/0009-0008-2425-1310
Department of Pharmacy, International Islamic University Chittagong, Bangladesh.
Md. Sifat Foysal
https://orcid.org/0009-0007-0917-2529
Department of Pharmacy, International Islamic University Chittagong, Bangladesh.
Jannatul Naima
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan; Department of Pharmacy, University of Chittagong, Bangladesh.
Sadab Sipar Ibban
sadabibban@gmail.com
https://orcid.org/0000-0002-6961-2422
Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan.

The Bangladeshi medicinal plant Leea aequata is utilized for many health ailments due to its phenolics and flavonoids; however, its bioactive qualities are unknown. An Ethanolic Extract of Leea aequata (EELA) was tested for antioxidant activity in a controlled lab study. It was also tested on rodents for feelings of depression and anxiety. Hole Board Test (HBT) and Elevated Plus Maze (EPM) assessed anxiolytic activity in intact mice. The Forced Swim Test (FST) and Tail Suspension Test (TST) assessed antidepressant action through immobility. DPPH scavenging, total phenolic, and total flavonoid assays assessed in vitro antioxidant capabilities. In the in vitro DPPH scavenging activity model, the half-Inhibition Concentration (IC50) of the plant sample for free radicals is 323.88 μg/mL, which is significant compared to that of ascorbic acid (759.03 μg/mL). The Total Phenol Content (TPC) of 25.78±3.75 Gallic Acid Equivalent (GAE) mg/g of dry extract and the Total Flavonoid Content (TFC) of 20.19 mg Catechin Equivalent (CAE) per gram of dry extract in the Leea aequata extract were found to be substantial. In the in vivo anxiolytic activity model, EELA showed substantial (p<0.01) anxiolytic efficacy at 400 mg/kg in the EPM test. The test extract's anxiolytic action is shown by the open arm's decreased entry at 400 mg/kg (81.33±13.96). Increased head dipping with strong anxiolytic effects at 400 mg/kg (27±4.04) (p<0.0001) was observed in HBT. In TST, EELA showed greater antidepressant effectiveness at 200 mg/kg (64.33±6.58). In the FST, EELA at 200 mg/kg had the strongest anti-depressant effect (p<0.0001) due to its short immobility period. These results suggest that L. aequata has antioxidant and neuropharmacological properties and is a major antioxidant source. According to considerable research, Leea aequata may reduce oxidative stress, anxiety, and depression.

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Biswas NN, Acharzo AK, Anamika S, et al. Screening of natural bioactive metabolites and investigation of antioxidant, antimicrobial, antihyperglycemic, neuropharmacological, and cytotoxicity potentials of Litsea polyantha Juss. ethanolic root extract. Evidence-based Complement Altern Med. 2017;2017:3701349. DOI: https://doi.org/10.1155/2017/3701349
Sharif S, Shahriar M, Haque A, et al. In-vitro antioxidant activities , anti-nociceptive and neuropharmacological activities of Polygonum hydropiper. Journal of Biology, Agriculture and Healthcare. 2013;3:61-72.
Burki S, Mehjabeen, Burki ZG, et al. Phytochemical screening, antioxidant, and in vivo neuropharmacological effect of Monotheca buxifolia (Falc.) barks extract. Pak J Pharm Sci. 2018;31:1519-28.
Mukherjee PK, Houghton PJ. The worldwide phenomenon of increased use of herbal products: opportunities and threats. Eval Herb Med Prod Pharm Press; London, UK; 2009.
Verma S, Singh SP. Current and future status of herbal medicines. Vet World. 2008;1:347. DOI: https://doi.org/10.5455/vetworld.2008.347-350
Rahman MA, Imran T bin, Islam S. Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi J Biol Sci. 2013;20:213-25. DOI: https://doi.org/10.1016/j.sjbs.2012.11.007
Chanwitheesuk A, Teerawutgulrag A, Rakariyatham N. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem. 2005;92:491-7. DOI: https://doi.org/10.1016/j.foodchem.2004.07.035
Patel V, Patel P, Kajal SS. Antioxidant activity of some selected medicinal plants in western region of India. Available from: https://www.idosi.org/abr/4(1)/5.pdf
Hayes JD, Dinkova-Kostova AT, Tew KD. Oxidative stress in cancer. Cancer Cell. 2020;38:167-97. DOI: https://doi.org/10.1016/j.ccell.2020.06.001
Vassalle C, Petrozzi L, Botto N, et al. Oxidative stress and its association with coronary artery disease and different atherogenic risk factors. J Intern Med. 2004;256:308-15. DOI: https://doi.org/10.1111/j.1365-2796.2004.01373.x
Vinson JA. Oxidative stress in cataracts. Pathophysiology. 2006;13:151-62. DOI: https://doi.org/10.1016/j.pathophys.2006.05.006
Wei W, Liu Q, Tan Y, et al. Oxidative stress, diabetes, and diabetic complications. Hemoglobin. 2009;33:370-7. DOI: https://doi.org/10.3109/03630260903212175
Quiñonez-Flores CM, González-Chávez SA, Del Río Nájera D, Pacheco-Tena C. Oxidative stress relevance in the pathogenesis of the rheumatoid arthritis: a systematic review. Biomed Res Int. 2016;2016:6097417. DOI: https://doi.org/10.1155/2016/6097417
Van der Ven A, Boers GHJ. Oxidative stress in immunodeficiency. Eur J Clin Invest. 1997;27:731-2. DOI: https://doi.org/10.1046/j.1365-2362.1997.1890739.x
Junqueira VBC, Barros SBM, Chan SS, et al. Aging and oxidative stress. Mol Aspects Med. 2004;25:5-16. DOI: https://doi.org/10.1016/j.mam.2004.02.003
Ibe S, Fujii Y, Otobe K. Studies on in vitro antioxidant activities of methanol extract of Mucuna pruriens (Fabaceae) seeds. Eur Bull Drug Res. 2005;13:31-9.
Khatun A, Rahman M, Rahman MM, et al. Antioxidant, antinociceptive and CNS activities of Viscum orientale and high sensitive quantification of bioactive polyphenols by UPLC. Front Pharmacol. 2016;7:1-12. DOI: https://doi.org/10.3389/fphar.2016.00176
Jayaprakasha GK, Selvi T, Sakariah KK. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Res Int. 2003;36:117-22. DOI: https://doi.org/10.1016/S0963-9969(02)00116-3
Ali S, Al S, Sayem J, Quah Y, et al. Neuropharmacological activities of Homalomena aromatica leaves using experimental and in silico approaches. Molecules. 2021;26:975. DOI: https://doi.org/10.3390/molecules26040975
Asif HM, Hayee A, Aslam MR, et al. Dose-dependent, antidepressant, and anxiolytic effects of a traditional medicinal plant for the management of behavioral dysfunctions in animal models. Dose-Response. 2019;17:1-6. DOI: https://doi.org/10.1177/1559325819891262
Hoyer J, Beesdo K, Gloster AT, et al. Worry exposure versus applied relaxation in the treatment of generalized anxiety disorder. Psychother Psychosom. 2009;78:106-15. DOI: https://doi.org/10.1159/000201936
Mohr DC, Tomasino KN, Lattie EG, et al. Intellicare: an eclectic, skills-based app suite for the treatment of depression and anxiety. J Med Internet Res. 2017;19:1-14. DOI: https://doi.org/10.2196/jmir.6645
Rakib A, Ahmed S, Islam MA, et al. Pharmacological studies on the antinociceptive, anxiolytic and antidepressant activity of Tinospora crispa. Phyther Res. 2020;34:2978-84. DOI: https://doi.org/10.1002/ptr.6725
Tun NL, Hu DB, Xia MY, et al. Chemical constituents from ethanoic extracts of the aerial parts of Leea aequata L., a traditional folk medicine of Myanmar. Nat Products Bioprospect. 2019;9:243-9. DOI: https://doi.org/10.1007/s13659-019-0209-y
S.K Jain, N. Manikpuri, Kujur M. Antibacterial activity of seeds, stems and roots of Leea aequata. 2010;7:453-6.
Rahim A, Mostofa MG, Sadik MG, et al. The anticancer activity of two glycosides from the leaves of Leea aequata L. Nat Prod Res. 2021;35:5867-71. DOI: https://doi.org/10.1080/14786419.2020.1798661
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci Technol. 1995;28:25-30. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
Barua N, Ibn Aziz MA, Tareq AM, et al. In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.). Biochem Biophys Reports. 2020;23:100772. DOI: https://doi.org/10.1016/j.bbrep.2020.100772
Škerget M, Kotnik P, Hadolin M, et al. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem. 2005;89:191-8. DOI: https://doi.org/10.1016/j.foodchem.2004.02.025
Sen S, De B, Devanna N, Chakraborty R. Total Phenolic, Total Flavonoid Content, and antioxidant capacity of the leaves of Meyna spinosa Roxb., an Indian medicinal plant. Chin J Nat Med. 2013;11:149-57. DOI: https://doi.org/10.1016/S1875-5364(13)60042-4
Tareq AM, Farhad S, Uddin ABMN, Hoque M. Chemical profiles, pharmacological properties, and in silico studies provide new insights on Cycas pectinata. Heliyon. 2020;6:e04061. DOI: https://doi.org/10.1016/j.heliyon.2020.e04061
Babar ZM, Jaswir I, Tareq AM, et al. In vivo anxiolytic and in vitro anti-inflammatory activities of water-soluble extract (WSE) of Nigella sativa (L.) seeds. Nat Prod Res. 2021;35:2793-8. DOI: https://doi.org/10.1080/14786419.2019.1667348
Aziz MAI, Barua N, Tareq AM, et al. Possible neuropharmacological effects of Adenia trilobata (Roxb.) in the Swiss albino mice model. Future Journal of Pharmaceutical Sciences. 2020;6:72. DOI: https://doi.org/10.1186/s43094-020-00102-5
Rahman T, Hosen I, Islam MMT, Shekhar HU. Oxidative stress and human health. Adv Biosci Biotechnol. 2012;03:997-1019. DOI: https://doi.org/10.4236/abb.2012.327123
Masudur S, Dewan R, Das A, Science N. Investigation of in vitro thrombolytic potential and phytochemical nature of Crinum latifolium L. leaves growing in coastal region of Bangladesh. International Journal of Biological & Pharmaceutical Research. 2013;4:1-7.
Rakib A, Ahmed S, Islam MA, et al. Antipyretic and hepatoprotective potential of Tinospora crispa and investigation of possible lead compounds through in silico approaches. Food Sci Nutr. 2020;8:547-56. DOI: https://doi.org/10.1002/fsn3.1339
Abbas ZK, Saggu S, Sakeran MI, et al. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves. Saudi J Biol Sci. 2014;22:322-6. DOI: https://doi.org/10.1016/j.sjbs.2014.11.015
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process. 2011;89:217-33. DOI: https://doi.org/10.1016/j.fbp.2010.04.008
Jadid N, Hidayati D, Hartanti SR, et al. Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. using DPPH assay. AIP Conf Proc. 2017;1854: 020019. DOI: https://doi.org/10.1063/1.4985410
Tareq AM, Sohel M, Uddin M, et al. Possible neuropharmacological effects of apis cerana indica beehive in the Swiss Albino mice. J Adv Biotechnol Exp Ther. 2020;3:128-34. DOI: https://doi.org/10.5455/jabet.2020.d117
Nic Dhonnchadha BÁ, Bourin M, Hascoët M. Anxiolytic-like effects of 5-HT2 ligands on three mouse models of anxiety. Behav Brain Res. 2003;140:203-14. DOI: https://doi.org/10.1016/S0166-4328(02)00311-X
Brown GR, Nemes C. The exploratory behaviour of rats in the hole-board apparatus: Is head-dipping a valid measure of neophilia? Behav Processes. 2008;78:442-8. DOI: https://doi.org/10.1016/j.beproc.2008.02.019
Walf AA, Frye CA. The use of the Elevated Plus Maze as an assay of anxiety-related behavior in rodents. Nat Protoc. 2007;2:322-8. DOI: https://doi.org/10.1038/nprot.2007.44
Porsolt RD, Anton G, Blavet N, Jalfre M. Behavioural despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol. 1978;47:379-91. DOI: https://doi.org/10.1016/0014-2999(78)90118-8

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Evaluation of antioxidant and neuropharmacological properties of Leea aequata leaves. (2024). Infectious Diseases and Herbal Medicine, 5(1). https://doi.org/10.4081/idhm.2024.390
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