Chimerism analysis after allogeneic hematopoietic stem cell transplantation: standardization and quality assurance
https://doi.org/10.4081/wpph.2023.9640
Accepted: 7 February 2023
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Background: the European Federation of Immunogenetics excellence accreditation awarded to the Transfusion Medicine Complex Structure of the “SS. Antonio and Biagio e Cesare Arrigo” Hospital in the year 2000, requires all laboratories working in the field of hematopoietic stem cell transplantation to meet specific standards for guaranteeing a good level of service quality. Objectives: to evaluate the performance of the new Applied Biosystems™ SeqStudio™ Genetic Analyzer by validating the PowerPlex® 16 HS System kit (Promega Italia Srl, Milan, Italy), at the Transfusion Medicine Laboratory of the “SS. Antonio e Biagio and Cesare Arrigo” Hospital of Alessandria. Materials and Methods: three different studies were conducted: sensitivity, mixture, reproducibility, using DNA from hematology patients undergoing allogeneic hematopoietic stem cell transplantation and DNA from blood donors. Results: the sensitivity studies using the AB SeqStudio genetic analyzer proved to be more sensitive than those used previously (AB 3130), while the values obtained in the mixture studies overlapped with the expected values and the results obtained in the reproducibility studies were replicable and the same correlation exists in the tests performed on both instruments. Conclusions: the AB SeqStudio genetic analyzer through the PowerPlex® 16 HS System kit demonstrates excellent performance in accuracy and reproducibility of results and is a better performing instrument in sensitivity than the previous analyzer.
Harmer A, Mascaretti L, Petershofen E. Accreditation of histocompatibility and immunogenetics laboratories: Achievements and future prospects from the European Federation for Immunogenetics Accreditation Programme. HLA. 2018. DOI: https://doi.org/10.1111/tan.13289
Kanate AS, Majhail NS, Savani BN, et al. Indications for Hematopoietic Cell Transplantation and Immune Effector Cell Therapy: Guidelines from the American Society for Transplantation and Cellular Therapy. Biol Blood Marrow Transplant J Am Soc Blood Marrow Transplant. 2020;26:1247–56. DOI: https://doi.org/10.1016/j.bbmt.2020.03.002
Goldman JM. A special report: bone marrow transplants using volunteer donors--recommendations and requirements for a standardized practice throughout the world--1994 update. The WMDA Executive Committee. Blood. 1994;84:2833–9. DOI: https://doi.org/10.1182/blood.V84.9.2833.2833
Bazinet A, Popradi G. A general practitioner’s guide to hematopoietic stem-cell transplantation. Curr Oncol Tor Ont. 2019;26:187–91. DOI: https://doi.org/10.3747/co.26.5033
Blouin AG, Ye F, Williams J, Askar M. A practical guide to chimerism analysis: Review of the literature and testing practices worldwide. Hum Immunol. 2021;82:838–49. DOI: https://doi.org/10.1016/j.humimm.2021.07.013
Clark JR, Scott SD, Jack AL, et al. Monitoring of chimerism following allogeneic haematopoietic stem cell transplantation (HSCT): technical recommendations for the use of short tandem repeat (STR) based techniques, on behalf of the United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group. Br J Haematol. 2015;168:26–37. DOI: https://doi.org/10.1111/bjh.13073
Bader P, Niethammer D, Willasch A, et al. How and when should we monitor chimerism after allogeneic stem cell transplantation? Bone Marrow Transplant. 2005;35:107–19. DOI: https://doi.org/10.1038/sj.bmt.1704715
Clemente I, Goncalo A, Faria C, et al. Relevance of Chimerism Analysis After Allogeneic Stem Cell Transplantation. Transplant Proc. 2017;49:890–2. DOI: https://doi.org/10.1016/j.transproceed.2017.01.065
Delie A, Verlinden A, Beel K, et al. Use of chimerism analysis after allogeneic stem cell transplantation: Belgian guidelines and review of the current literature. Acta Clin Belg. 2021;76:500–8. DOI: https://doi.org/10.1080/17843286.2020.1754635
European Federation for Immunogenetics. STANDARDS FOR HISTOCOMPATIBILITY & IMMUNOGENETICS TESTING. 2020; Version 8.0. available from: https://efi-web.org/fileadmin/Efi_web/Standardv8_280819.pdf
Copyright (c) 2023 Giuseppina Balza, Menada Gardalini, Roberta Neri, Lia Mele, Graziella Peretti, Roberta Di Matteo, Denise Gatti, Tatiana Bolgeo, Antonio Maconi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Downloads
Citations
