Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy applied to the analysis of fibers and particles in tissues from colon adenocarcinomas
https://doi.org/10.4081/wpph.2023.9586
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Authors
- Alessandro Croce SSD Research Laboratories, Department of Science and Technological Innovation, University of Eastern Piedmont, Alessandria, Italy. https://orcid.org/0000-0001-8506-7535
- Marinella Bertolotti SSD Research Laboratories, Research and Innovation Department (DAIRI), Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy. https://orcid.org/0000-0003-2152-4930
- Stefania Crivellari SSD Research Laboratories, Research and Innovation Department (DAIRI), Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy. https://orcid.org/0000-0002-1997-8792
- Marco Amisano SC General Surgery, Presidio Ospedaliero S. Spirito, Casale Monferrato, Local Health Authority of Alessandria, Italy.
- Elisabetta Nada SC General Surgery, Presidio Ospedaliero S. Spirito, Casale Monferrato, Local Health Authority of Alessandria, Italy.
- Federica Grosso SSD Mesothelioma, Azienda Ospedaliera “SS. Antonio e Biagio e Cesare Arrigo”, Alessandria, Italy, Italy. https://orcid.org/0000-0002-6843-3369
- Laura Cagna SSD Research Laboratories, Department of Science and Technological Innovation, University of Eastern Piedmont, Alessandria, Italy.
- Caterina Rinaudo Department for Sustainable Development and Ecological Transaction, University of Eastern Piedmont, Vercelli, Italy.
- Giorgio Gatti Department for Sustainable Development and Ecological Transaction, University of Eastern Piedmont, Vercelli, Italy, Italy.
- Antonio Maconi Research Training Innovation Infrastructure, Research and Innovation Department (DAIRI), Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy. https://orcid.org/0000-0002-1904-7277
Background: The mineral phases regulated as “asbestos” have a well-known role in disease development in the respiratory tract (e.g. mesothelioma, pulmonary carcinoma, asbestosis), but it is not clear their role in cancer development in other body sites, as in colon-rectum tract. Materials and Methods: In this work, seven colon tissues (healthy and neoplastic portions and an area “bridge” between them) from patients affected by colon adenocarcinoma – and living in a highly asbestos-polluted area - have been digested and the inorganic residual components collected on polycarbonate filters analyzed by means of Scanning Electron Microscopy (SEM) with annexed an Energy Dispersive Spectroscopy (EDS) for elemental chemical analysis. Results: The obtained results allow us to characterize serpentine phases in two of the seven analyzed patients. Moreover, calcium phosphate phases and other metal-rich particles have been observed inside the samples. Conclusions: SEM/EDS allowed us to morphologically observe and chemically analyze not only asbestos phases, but also other inorganic particles inside tissues deriving from colon adenocarcinomas.
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CRT foundationCopyright (c) 2023 Alessandro Croce, Marinella Bertolotti, Stefania Crivellari, Marco Amisano, Elisabetta Nada, Federica Grosso, Laura Cagna, Caterina Rinaudo, Giorgio Gatti, Antonio Maconi
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