Fecal microbiota transplantation: fast parasites diagnosis by multiplex PCR


Published: 4 May 2021
Abstract Views: 1085
PDF (Italiano): 190
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Authors

  • Omar Mura Dipartimento di Scienze e Innovazione Tecnologica, Universit  del Piemonte Orientale “Amedeo Avogadro”, Alessandria, Italy.
  • Elisa Gamalero Dipartimento di Scienze e Innovazione Tecnologica, Universit  del Piemonte Orientale “Amedeo Avogadro”, Alessandria, Italy.
  • Luigi Di Matteo SC Microbiologia e Virologia, AO “SS Antonio e Biagio e Cesare Arrigo”, Alessandria, Italy.
  • Andrea Rocchetti SC Microbiologia e Virologia, AO “SS Antonio e Biagio e Cesare Arrigo”, Alessandria, Italy.

Objectives: Evaluation of the analytical performance of the Novodiag  Stool Parasites biomolecular assay for the detection of intestinal parasites in faeces of the faecal microbiota transplant donor. Methodology: Comparison between the gold standard for the diagnosis of intestinal parasites, light microscopy, and the Novodiag  Stool Parasites biomolecular assay through the use of 22 fecal samples. Results: Twelve out of 22 samples were positive on microscopic examination. In order to assess the overlapping between the results obtained through parasitological examination and the molecular methodology, all samples were tested to the Novodiag  Stool Parasites assay. Positive samples detected by biomolecular assay were 11 out of 22. Conclusion: The preliminary evaluation of the analytical performance of the Novodiag  Stool Parasites kit can be considered promising. The Novodiag  Stool Parasites kit can be effectively used as a tool in the fast diagnosis of intestinal parasitoses on the faecal microbiota transplant donor faeces.


Mura, O., Gamalero, E., Di Matteo, L., & Rocchetti, A. (2021). Fecal microbiota transplantation: fast parasites diagnosis by multiplex PCR. Working Paper of Public Health, 9(1). https://doi.org/10.4081/wpph.2021.9311

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