Use of multi-locus sequencing typing as identification method for the food-borne pathogen Listeria monocytogenes: a review

Sonia Lamon; Domenico Meloni; Simonetta Gianna Consolati; Anna Mureddu; Rina Mazzette

doi:10.4081/vsd.2015.5753

Use of multi-locus sequencing typing as identification method for the food-borne pathogen Listeria monocytogenes: a review

https://doi.org/10.4081/vsd.2015.5753

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Vol. 5 No. 1 (2015)

Submitted: 9 December 2014
Accepted: 30 December 2014

Published: 27 January 2015

Listeria monocytogenes, MLST, identification, typing

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Authors

Sonia Lamon Department of Veterinary Medicine, University of Sassari, Italy.
Domenico Meloni
dmeloni@uniss.it
Department of Veterinary Medicine, University of Sassari, Italy.
Simonetta Gianna Consolati Department of Veterinary Medicine, University of Sassari, Italy.
Anna Mureddu Department of Veterinary Medicine, University of Sassari, Italy.
Rina Mazzette Department of Veterinary Medicine, University of Sassari, Italy.

Listeria monocytogenes is an ubiquitous, intracellular pathogen which has been implicated within the past decade as the causative organism in several outbreaks of foodborne diseases. In this review, a new approach to molecular typing primarily designed for global epidemiology has been described: multi-locus sequencing typing (MLST). This approach is novel, in that it uses data that allow the unambiguous characterization of bacterial strains via the Internet. Our aim is to present the currently available selection of references on L. monocytogenes MLST detection methods and to discuss its use as gold standard to L. monocytogenes subtyping method.

Supporting Agencies

Lamon, S., Meloni, D., Consolati, S. G., Mureddu, A., & Mazzette, R. (2015). Use of multi-locus sequencing typing as identification method for the food-borne pathogen Listeria monocytogenes: a review. Veterinary Science Development, 5(1). https://doi.org/10.4081/vsd.2015.5753