Distribution of Mycobacterium leprae genotypes from Surabaya and Bandung Clinical Isolates by Multiple Locus Variable Number of Tandem Repeat Analysis


https://doi.org/10.4081/dr.2019.8017
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Authors

  • Cita Rosita Sigit Prakoeswa
    drcita.rosita@gmail.com
    Department of Dermatology and Venereology, Faculty of Medicine Universitas Airlangga, Dr Soetomo Hospital, Surabaya, Indonesia.
  • Bayu Bijaksana Rumondor Faculty of Medicine, Universitas Airlangga, Dr Soetomo Hospital, Surabaya, Indonesia.
  • Lina Damayanti Department of Dermatology and Venereology, Faculty of Medicine Padjadjaran University, Dr Hasan Sadikin Hospital, Bandung, Indonesia.
  • Muljaningsih Sasmojo Department of Dermatology and Venereology, Faculty of Medicine Padjadjaran University, Dr Hasan Sadikin Hospital, Bandung, Indonesia.
  • Dinar Adriaty Leprosy Study Group, Tropical Diseases Center, Universitas Airlangga, Surabaya, Indonesia.
  • Medhi Denisa Alinda Department of Dermatology and Venereology, Faculty of Medicine Universitas Airlangga, Dr Soetomo Hospital, Surabaya, Indonesia.
  • Ratna Wahyuni Leprosy Study Group, Tropical Diseases Center, Universitas Airlangga, Surabaya, Indonesia.
  • M. Yulianto Listiawan Department of Dermatology and Venereology, Faculty of Medicine Universitas Airlangga, Dr Soetomo Hospital, Surabaya, Indonesia.
  • Indropo Agusni Department of Dermatology and Venereology, Faculty of Medicine Universitas Airlangga, Dr Soetomo Hospital, Surabaya, Indonesia.
  • Shinzo Izumi Leprosy Study Group, Tropical Diseases Center, Universitas Airlangga, Surabaya, Indonesia.

Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis has been proposed as a means of genotyping for tracking leprosy transmission due to tandem repeats’ potential as genetic markers to differentiate M. leprae strains. However, characteristics of polymorphism can vary depending on the population. This study aimed to compare the copy number of repeats in four genetic markers: TTC, AC8a, AC9 and 6-7 in leprosy patients from Surabaya and Bandung. Twenty three patients from Dr. Soetomo General Hospital and 21 from Hasan Sadikin Hospital were recruited. Multiple locus VNTR analysis was applied using total DNA extracts from Slit Skin Smear (SSS). From Surabaya, 7 samples showed the same copy number of four genetic markers (TTC=15; AC8a=10; AC9=10 and 6-7=6) and 2 showed another (TTC=16; AC8a=10; AC9=11 and 6-7=6); as for samples from Bandung, 2 showed the same copy number (TTC=15; AC8a=8; AC9=10 and 6-7=8) and 2 showed another (TTC=16; AC8a=10; AC9=11 and 6-7=6). The multiple locus VNTR analysis showed two identical M. leprae VNTR profiles from Bandung and Surabaya which supports the use of VNTR loci for transmission studies.