An automated continuous monitoring system: a useful tool for monitoring neuronal differentiation of human embryonic stem cells


https://doi.org/10.4081/scs.2011.e10
Vol. 1 No. 1 (2011)
Submitted: 27 May 2011
Accepted: 18 July 2011
Published: 22 August 2011
human embryonic stem cells, neural differentiation, automated imaging, machine vision.
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Authors

  • Tuomas Tapani Huttunen Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland.
  • Maria Sundberg Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland.
  • Harri Pihlajamäki Centre for Military Medicine, Helsink, Finland.
  • Riitta Suuronen Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital; Department of Eye, Ear and Oral Diseases, Tampere University Hospital, Tampere; Department of Biomedical Engineering, Tampere University of Technology, Tampere, Finland.
  • Heli Skottman Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland.
  • Riikka Äänismaa Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland.
  • Susanna Narkilahti
    susanna.narkilahti@uta.fi
    Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Tampere, Finland.
The currently used cell culturing and differentiation procedures are both time- and laborintensive. Automation of some of these procedures will increase the efficiency of commonly used cell differentiation protocols. We used a particular cell culture platform to rapidly and efficiently screen the neuronal differentiation of human embryonic stem cells (hESC). Continuous live monitoring and analysis of non-labeled cells using this system allowed us to characterize neuronal populations over the entire neuronal differentiation process. The differentiation of individual cells from early progenitor cells to neurons and glial cells could be monitored continuously using this system with sub-confluent cell cultures. The imaged data was collected and analyzed with a specially designed cell recognition protocol, which resulted in a quantitative neuronal cell count. The analysis results were confirmed using conventional laboratory methods such as manual counting and flow cytometry. Our findings suggest that an automated culture platform combined with automated monitoring and analysis systems is a reliable method for developing enhanced cell differentiation procedures or as part of an automated quality control system for existing protocols.

Supporting Agencies


Huttunen, T. T., Sundberg, M., Pihlajamäki, H., Suuronen, R., Skottman, H., Äänismaa, R., & Narkilahti, S. (2011). An automated continuous monitoring system: a useful tool for monitoring neuronal differentiation of human embryonic stem cells. Stem Cell Studies, 1(1), e10. https://doi.org/10.4081/scs.2011.e10

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