https://doi.org/10.4081/pb.2012.e7

Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.

Vol. 3 No. 1 (2012)
Submitted: 30 September 2011
Accepted: 23 July 2012
Published: 10 December 2012
Artemisia annua L., tissue cultured leaves, total RNA extraction, PCR
Abstract Views: 3166
PDF: 415
HTML: 49
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Suganthi Appalasamy
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
Ning Shu Ping
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
Arvind Bhatt
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
Ahmad Sofiman Othman
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
Nad-Ali Babaeian Jelodar
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
Chan Lai Keng
merrilynchan@gmail.com
Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, University of Sains Malaysia, Penang, Malaysia.
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant. This innovative protocol ensures a consistently high quantity and good quality of total RNA without any contamination of polyphenols, polysaccharides and proteins. The total RNA obtained is also free of fungal RNA even when extracted from fungal infested plants. The extraction buffer used in the proposed modified protocol was made up of non-hazardous chemicals. High concentrations of polyphenols of A. annua L. could be successfully eliminated and the prepared total RNA could be used for downstream reactions.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC

Supporting Agencies

How to Cite

Appalasamy, S., Shu Ping, N., Bhatt, A., Othman, A. S., Jelodar, N.-A. B., & Lai Keng, C. (2012). Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L. International Journal of Plant Biology, 3(1), e7. https://doi.org/10.4081/pb.2012.e7

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.